产品货号：
Z26962
中文名称：
SPHK1抗体
英文名称：
Anti-SPHK1 Antibody
产品规格：
50μl|100μl|150μl
发货周期：
1～3天
产品价格：
询价
| 抗体名称 | Anti-SPHK1 Antibody |
| 指标别名 | SK 1;sphingosine kinase 1;SPHK;SPHK1;SPHK1 phopho;SPK;SPK 1 |
| 克隆性 | Polyclonal |
| 检验物种 | human,mouse,rat |
| 应用范围 | WB,IHC,ICC/IF,FCM,ELISA |
| 基因名称 | SPHK1 |
| 抗体来源 | Rabbit |
| 抗体类型 | IgG |
| 免疫原 | E.coli-derived human SPHK1 recombinant protein (Position:R16-H267). |
| 实际分子量 | 45～50KD |
| 成分 | 500μg/mL antibody with PBS，0.02% NaN3,1mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500μg/mL |
| 产品形态 | 溶液 |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing. |
| 背景资料 | SPHK1(Sphingosine Kinase 1),is an enzyme that in humans is encoded by the SPHK1 gene.Melendez et al.(2000) mapped the SPHK1 gene to chromosome 17q25.2 based on sequence identity with ESTs mapped to this region.Kohama et al.(1998) demonstrated that recombinant mouse Sphk1 can specifically phosphorylate D-erythro-sphingosine and that D,L-threo-dihydrosphingosine and N,N-dimethylsphingosine can act as competitive inhibitors of recombinant Sphk1.Pitson et al.(2000) found that recombinant SPHK1 and endogenous SPHK1 purified from placenta had identical enzymatic characteristics,suggesting posttranslational modification does not effect functional properties. |
| Uniprot ID | Q9NYA1 |
| 推荐配套试剂 | Biorab recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ZN1926) for Western blot. |
| 基因名全称 | sphingosine kinase 1 |
| 蛋白名全称 | Sphingosine kinase 1 |
| 推荐稀释比 | Western blot (WB) 1:500～2000 Immunohistochemistry (IHC) 1:50～400 Immunocytochemistry/Immunofluorescence (ICC/IF) 1:50～400 Flow cytometry (FCM) 1～3μg/1×106 cells Enzyme linked immunosorbent assay (ELISA) 1:100～1000 (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
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